Single cell transcriptomics of ABEDINIUM reveals a new early-branching dinoflagellate lineage.
Identifieur interne : 000044 ( Main/Exploration ); précédent : 000043; suivant : 000045Single cell transcriptomics of ABEDINIUM reveals a new early-branching dinoflagellate lineage.
Auteurs : Elizabeth C. Cooney [Canada] ; Noriko Okamoto [Canada] ; Anna Cho [Canada] ; Elisabeth Hehenberger [Canada, Allemagne] ; Thomas A. Richards [Royaume-Uni] ; Alyson E. Santoro [États-Unis] ; Alexandra Z. Worden [Allemagne] ; Brian S. Leander [Canada] ; Patrick J. Keeling [Canada]Source :
- Genome biology and evolution [ 1759-6653 ] ; 2020.
Abstract
Dinoflagellates possess many cellular characteristics with unresolved evolutionary histories. These include nuclei with greatly expanded genomes and chromatin packaged using histone-like proteins and dinoflagellate-viral nucleoproteins instead of histones, highly reduced mitochondrial genomes with extensive RNA editing, a mix of photosynthetic and cryptic secondary plastids, and tertiary plastids. Resolving the evolutionary origin of these traits requires understanding their ancestral states and early intermediates. Several early-branching dinoflagellate lineages are good candidates for such reconstruction, however these cells tend to be delicate and environmentally sparse, complicating such analyses. Here, we employ transcriptome sequencing from manually isolated and microscopically documented cells to resolve the placement of two cells of one such genus, Abedinium, collected by Remotely Operated Vehicle (ROV) in deep waters off the coast of Monterey Bay, California, USA. One cell corresponds to the only described species, A. dasypus, while the second cell is distinct and formally described as Abedinium folium, sp. nov. Abedinium has classically been assigned to the early-branching dinoflagellate subgroup Noctilucales, which is weakly supported by phylogenetic analyses of small subunit ribosomal RNA (SSU rRNA), the single characterized gene from any member of the order. However, an analysis based on 221 proteins from the transcriptome places Abedinium as a distinct lineage, separate from and basal to Noctilucales and the rest of the core dinoflagellates. The transcriptome also contains evidence of a cryptic plastid functioning in the biosynthesis of isoprenoids, iron-sulfur clusters, and heme, a mitochondrial genome with all three expected protein-coding genes (cob, cox1, and cox3), and the presence of some but not all dinoflagellate-specific chromatin packaging proteins.
DOI: 10.1093/gbe/evaa196
PubMed: 33045041
Affiliations:
- Allemagne, Canada, Royaume-Uni, États-Unis
- Angleterre, Californie, Colombie-Britannique , Oxfordshire, Schleswig-Holstein
- Kiel, Oxford, Vancouver
- Université d'Oxford, Université de la Colombie-Britannique
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Single cell transcriptomics of ABEDINIUM reveals a new early-branching dinoflagellate lineage.</title><author><name sortKey="Cooney, Elizabeth C" sort="Cooney, Elizabeth C" uniqKey="Cooney E" first="Elizabeth C" last="Cooney">Elizabeth C. Cooney</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Okamoto, Noriko" sort="Okamoto, Noriko" uniqKey="Okamoto N" first="Noriko" last="Okamoto">Noriko Okamoto</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Cho, Anna" sort="Cho, Anna" uniqKey="Cho A" first="Anna" last="Cho">Anna Cho</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Hehenberger, Elisabeth" sort="Hehenberger, Elisabeth" uniqKey="Hehenberger E" first="Elisabeth" last="Hehenberger">Elisabeth Hehenberger</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation></author><author><name sortKey="Richards, Thomas A" sort="Richards, Thomas A" uniqKey="Richards T" first="Thomas A" last="Richards">Thomas A. Richards</name><affiliation wicri:level="4"><nlm:affiliation>Zoology Department, University of Oxford, Oxford, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Zoology Department, University of Oxford, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author><author><name sortKey="Santoro, Alyson E" sort="Santoro, Alyson E" uniqKey="Santoro A" first="Alyson E" last="Santoro">Alyson E. Santoro</name><affiliation wicri:level="2"><nlm:affiliation>Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Worden, Alexandra Z" sort="Worden, Alexandra Z" uniqKey="Worden A" first="Alexandra Z" last="Worden">Alexandra Z. Worden</name><affiliation wicri:level="3"><nlm:affiliation>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Kiel University, Christian-Albrechts-Platz 4, Kiel, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Kiel University, Christian-Albrechts-Platz 4, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation></author><author><name sortKey="Leander, Brian S" sort="Leander, Brian S" uniqKey="Leander B" first="Brian S" last="Leander">Brian S. Leander</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Zoology, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Zoology, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Keeling, Patrick J" sort="Keeling, Patrick J" uniqKey="Keeling P" first="Patrick J" last="Keeling">Patrick J. Keeling</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33045041</idno><idno type="pmid">33045041</idno><idno type="doi">10.1093/gbe/evaa196</idno><idno type="wicri:Area/Main/Corpus">000020</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000020</idno><idno type="wicri:Area/Main/Curation">000020</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000020</idno><idno type="wicri:Area/Main/Exploration">000020</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Single cell transcriptomics of ABEDINIUM reveals a new early-branching dinoflagellate lineage.</title><author><name sortKey="Cooney, Elizabeth C" sort="Cooney, Elizabeth C" uniqKey="Cooney E" first="Elizabeth C" last="Cooney">Elizabeth C. Cooney</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Okamoto, Noriko" sort="Okamoto, Noriko" uniqKey="Okamoto N" first="Noriko" last="Okamoto">Noriko Okamoto</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Cho, Anna" sort="Cho, Anna" uniqKey="Cho A" first="Anna" last="Cho">Anna Cho</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Hehenberger, Elisabeth" sort="Hehenberger, Elisabeth" uniqKey="Hehenberger E" first="Elisabeth" last="Hehenberger">Elisabeth Hehenberger</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation></author><author><name sortKey="Richards, Thomas A" sort="Richards, Thomas A" uniqKey="Richards T" first="Thomas A" last="Richards">Thomas A. Richards</name><affiliation wicri:level="4"><nlm:affiliation>Zoology Department, University of Oxford, Oxford, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Zoology Department, University of Oxford, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author><author><name sortKey="Santoro, Alyson E" sort="Santoro, Alyson E" uniqKey="Santoro A" first="Alyson E" last="Santoro">Alyson E. Santoro</name><affiliation wicri:level="2"><nlm:affiliation>Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Worden, Alexandra Z" sort="Worden, Alexandra Z" uniqKey="Worden A" first="Alexandra Z" last="Worden">Alexandra Z. Worden</name><affiliation wicri:level="3"><nlm:affiliation>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Kiel University, Christian-Albrechts-Platz 4, Kiel, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Kiel University, Christian-Albrechts-Platz 4, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation></author><author><name sortKey="Leander, Brian S" sort="Leander, Brian S" uniqKey="Leander B" first="Brian S" last="Leander">Brian S. Leander</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Zoology, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Zoology, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Keeling, Patrick J" sort="Keeling, Patrick J" uniqKey="Keeling P" first="Patrick J" last="Keeling">Patrick J. Keeling</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></analytic><series><title level="j">Genome biology and evolution</title><idno type="eISSN">1759-6653</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dinoflagellates possess many cellular characteristics with unresolved evolutionary histories. These include nuclei with greatly expanded genomes and chromatin packaged using histone-like proteins and dinoflagellate-viral nucleoproteins instead of histones, highly reduced mitochondrial genomes with extensive RNA editing, a mix of photosynthetic and cryptic secondary plastids, and tertiary plastids. Resolving the evolutionary origin of these traits requires understanding their ancestral states and early intermediates. Several early-branching dinoflagellate lineages are good candidates for such reconstruction, however these cells tend to be delicate and environmentally sparse, complicating such analyses. Here, we employ transcriptome sequencing from manually isolated and microscopically documented cells to resolve the placement of two cells of one such genus, Abedinium, collected by Remotely Operated Vehicle (ROV) in deep waters off the coast of Monterey Bay, California, USA. One cell corresponds to the only described species, A. dasypus, while the second cell is distinct and formally described as Abedinium folium, sp. nov. Abedinium has classically been assigned to the early-branching dinoflagellate subgroup Noctilucales, which is weakly supported by phylogenetic analyses of small subunit ribosomal RNA (SSU rRNA), the single characterized gene from any member of the order. However, an analysis based on 221 proteins from the transcriptome places Abedinium as a distinct lineage, separate from and basal to Noctilucales and the rest of the core dinoflagellates. The transcriptome also contains evidence of a cryptic plastid functioning in the biosynthesis of isoprenoids, iron-sulfur clusters, and heme, a mitochondrial genome with all three expected protein-coding genes (cob, cox1, and cox3), and the presence of some but not all dinoflagellate-specific chromatin packaging proteins.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33045041</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1759-6653</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>12</Day></PubDate></JournalIssue><Title>Genome biology and evolution</Title><ISOAbbreviation>Genome Biol Evol</ISOAbbreviation></Journal><ArticleTitle>Single cell transcriptomics of ABEDINIUM reveals a new early-branching dinoflagellate lineage.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">evaa196</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/gbe/evaa196</ELocationID><Abstract><AbstractText>Dinoflagellates possess many cellular characteristics with unresolved evolutionary histories. These include nuclei with greatly expanded genomes and chromatin packaged using histone-like proteins and dinoflagellate-viral nucleoproteins instead of histones, highly reduced mitochondrial genomes with extensive RNA editing, a mix of photosynthetic and cryptic secondary plastids, and tertiary plastids. Resolving the evolutionary origin of these traits requires understanding their ancestral states and early intermediates. Several early-branching dinoflagellate lineages are good candidates for such reconstruction, however these cells tend to be delicate and environmentally sparse, complicating such analyses. Here, we employ transcriptome sequencing from manually isolated and microscopically documented cells to resolve the placement of two cells of one such genus, Abedinium, collected by Remotely Operated Vehicle (ROV) in deep waters off the coast of Monterey Bay, California, USA. One cell corresponds to the only described species, A. dasypus, while the second cell is distinct and formally described as Abedinium folium, sp. nov. Abedinium has classically been assigned to the early-branching dinoflagellate subgroup Noctilucales, which is weakly supported by phylogenetic analyses of small subunit ribosomal RNA (SSU rRNA), the single characterized gene from any member of the order. However, an analysis based on 221 proteins from the transcriptome places Abedinium as a distinct lineage, separate from and basal to Noctilucales and the rest of the core dinoflagellates. The transcriptome also contains evidence of a cryptic plastid functioning in the biosynthesis of isoprenoids, iron-sulfur clusters, and heme, a mitochondrial genome with all three expected protein-coding genes (cob, cox1, and cox3), and the presence of some but not all dinoflagellate-specific chromatin packaging proteins.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cooney</LastName><ForeName>Elizabeth C</ForeName><Initials>EC</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Okamoto</LastName><ForeName>Noriko</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hehenberger</LastName><ForeName>Elisabeth</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Richards</LastName><ForeName>Thomas A</ForeName><Initials>TA</Initials><AffiliationInfo><Affiliation>Zoology Department, University of Oxford, Oxford, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santoro</LastName><ForeName>Alyson E</ForeName><Initials>AE</Initials><AffiliationInfo><Affiliation>Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Worden</LastName><ForeName>Alexandra Z</ForeName><Initials>AZ</Initials><AffiliationInfo><Affiliation>Ocean Ecosystems Biology Unit, GEOMAR - Helmholtz Centre for Ocean Research Kiel, Duesternbrooker Weg 20, Kiel, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Kiel University, Christian-Albrechts-Platz 4, Kiel, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leander</LastName><ForeName>Brian S</ForeName><Initials>BS</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Zoology, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Keeling</LastName><ForeName>Patrick J</ForeName><Initials>PJ</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver V6T 1Z4, British Columbia, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Genome Biol Evol</MedlineTA><NlmUniqueID>101509707</NlmUniqueID><ISSNLinking>1759-6653</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Dinoflagellate evolution</Keyword><Keyword MajorTopicYN="N">cryptic plastid</Keyword><Keyword MajorTopicYN="N">noctilucoid</Keyword><Keyword MajorTopicYN="N">single cell transcriptomics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>08</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>12</Day><Hour>17</Hour><Minute>10</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33045041</ArticleId><ArticleId IdType="pii">5921181</ArticleId><ArticleId IdType="doi">10.1093/gbe/evaa196</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Canada</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Californie</li><li>Colombie-Britannique </li><li>Oxfordshire</li><li>Schleswig-Holstein</li></region><settlement><li>Kiel</li><li>Oxford</li><li>Vancouver</li></settlement><orgName><li>Université d'Oxford</li><li>Université de la Colombie-Britannique</li></orgName></list><tree><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Cooney, Elizabeth C" sort="Cooney, Elizabeth C" uniqKey="Cooney E" first="Elizabeth C" last="Cooney">Elizabeth C. Cooney</name></region><name sortKey="Cho, Anna" sort="Cho, Anna" uniqKey="Cho A" first="Anna" last="Cho">Anna Cho</name><name sortKey="Hehenberger, Elisabeth" sort="Hehenberger, Elisabeth" uniqKey="Hehenberger E" first="Elisabeth" last="Hehenberger">Elisabeth Hehenberger</name><name sortKey="Keeling, Patrick J" sort="Keeling, Patrick J" uniqKey="Keeling P" first="Patrick J" last="Keeling">Patrick J. Keeling</name><name sortKey="Leander, Brian S" sort="Leander, Brian S" uniqKey="Leander B" first="Brian S" last="Leander">Brian S. Leander</name><name sortKey="Leander, Brian S" sort="Leander, Brian S" uniqKey="Leander B" first="Brian S" last="Leander">Brian S. Leander</name><name sortKey="Okamoto, Noriko" sort="Okamoto, Noriko" uniqKey="Okamoto N" first="Noriko" last="Okamoto">Noriko Okamoto</name></country><country name="Allemagne"><region name="Schleswig-Holstein"><name sortKey="Hehenberger, Elisabeth" sort="Hehenberger, Elisabeth" uniqKey="Hehenberger E" first="Elisabeth" last="Hehenberger">Elisabeth Hehenberger</name></region><name sortKey="Worden, Alexandra Z" sort="Worden, Alexandra Z" uniqKey="Worden A" first="Alexandra Z" last="Worden">Alexandra Z. Worden</name><name sortKey="Worden, Alexandra Z" sort="Worden, Alexandra Z" uniqKey="Worden A" first="Alexandra Z" last="Worden">Alexandra Z. Worden</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Richards, Thomas A" sort="Richards, Thomas A" uniqKey="Richards T" first="Thomas A" last="Richards">Thomas A. Richards</name></region></country><country name="États-Unis"><region name="Californie"><name sortKey="Santoro, Alyson E" sort="Santoro, Alyson E" uniqKey="Santoro A" first="Alyson E" last="Santoro">Alyson E. Santoro</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/IronSulferCluV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000044 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000044 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= IronSulferCluV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:33045041
|texte= Single cell transcriptomics of ABEDINIUM reveals a new early-branching dinoflagellate lineage.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:33045041" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IronSulferCluV1
| This area was generated with Dilib version V0.6.38. |  |